Journal: OncoTargets and Therapy

Article Title: Chemotherapy induces ovarian cancer cell repopulation through the caspase 3-mediated arachidonic acid metabolic pathway

doi: 10.2147/ott.s150456

Figure Lengend Snippet: Figure 1 The PGE2 and AA level and the expression of FAK in the clinical samples before and after chemotherapy. Notes: (A) The AA and PGE2 levels of patient plasma samples were measured by ELISA. The PGE2-to-AA ratios of patient plasma samples before and after chemotherapy were compared. Three independent experiments were carried out. The results are expressed as the mean ± SD of three independent experiments, *P,0.05 compared with the control group. (B) Immunohistochemical staining of FAK and p-FAK in patient ovarian cancer samples before and after chemotherapy. (C) Levels of FAK and p-FAK were quantified according to immunohistochemical staining score. Abbreviations: PGE2, prostaglandin E2; AA, arachidonic acid; FAK, focal adhesion kinase; ELISA, enzyme-linked immunosorbent assay; SD, standard deviation; p-FAK, phosphorylated FAK; IHC, immunohistochemical.

Article Snippet: A human PGE 2 enzyme-linked immunosorbent assay (ELISA) kit and human AA ELISA kit were purchased from R&D Systems.

Techniques: Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control, Immunohistochemical staining, Staining, Standard Deviation

Journal: OncoTargets and Therapy

Article Title: Chemotherapy induces ovarian cancer cell repopulation through the caspase 3-mediated arachidonic acid metabolic pathway

doi: 10.2147/ott.s150456

Figure Lengend Snippet: Figure 4 The AA and PGE2 levels within the Transwell system. Notes: (A and B) The AA and PGE2 levels of the supernatants collected each day from the Transwell system during the 10-day incubation were measured by ELISA. (C) The PGE2-to-AA ratios were calculated. Three independent experiments were carried out. The results are expressed as the mean ± SD, n=3, *P,0.05 compared with the control group. Abbreviations: AA, arachidonic acid; PGE2, prostaglandin E2; ELISA, enzyme-linked immunosorbent assay; SD, standard deviation.

Article Snippet: A human PGE 2 enzyme-linked immunosorbent assay (ELISA) kit and human AA ELISA kit were purchased from R&D Systems.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Control, Standard Deviation

Journal: Journal of Advanced Research

Article Title: Characterization of immune features and discovery of potential biomarkers for ankylosing spondylitis using deep plasma proteomics

doi: 10.1016/j.jare.2025.05.052

Figure Lengend Snippet: Verification of potential biomarkers for AS. (A) Expression levels of SAA1, FERMT3, ILK, and TLN1 in plasma samples from active-stage AS patients, stable-stage AS patients, and healthy controls measured by proteomics. ROC curves for the individual protein (SAA1, FERMT3, ILK, and TLN1) as a predictor to classify AS patients from healthy controls (B), and active-stage AS patients from stable-stage AS patients (C). (D) ELISA analysis of SAA1, FERMT3, ILK, and TLN1 expressions in the plasma samples from an independent validation cohort of healthy controls (N, n = 24), active-stage AS (A, n = 27), and stable-stage AS patients (S, n = 28). Statistical analyses employed Wilcoxon rank-sum test for intergroup comparisons, with results reported as mean ± SEM. Significance thresholds based on BH-adjusted p -values were defined as: *, <0.05; **, <0.01; ***, <0.001.

Article Snippet: The human serum amyloid A (SAA1) ELISA kit (#OKIA00083) was purchased from Aviva Systems Biology Company (San Diego, CA, USA).

Techniques: Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Biomarker Discovery

Journal: Drug Design, Development and Therapy

Article Title: Beraprost sodium preconditioning prevents inflammation, apoptosis, and autophagy during hepatic ischemia-reperfusion injury in mice via the P38 and JNK pathways

doi: 10.2147/DDDT.S182292

Figure Lengend Snippet: BPS preconditioning inhibited TNF-α and IL-1β expressions. Notes: ( A ) Relative TNF- α and IL- 1β mRNA levels were determined by qRT-PCR. ( B ) Serum TNF-α and IL-1β were detected by ELISA. ( C ) TNF-α and IL-1β protein expressions in liver tissues at 8 hours after reperfusion are shown by immunohistochemical staining. Final evaluations were made using Image-Pro Plus 6.0 software to calculate the IODs of the positive staining area. ( D ) Western blot analysis of TNF-α and IL-1β protein levels. Gray values were determined by Odyssey software. Relative TNF-α and IL-1β levels were measured through relative changes of gray values. Data are presented as mean ± SD (n=6; * P <0.05 for IR vs sham; # P <0.05 for IR+BPS [50 µg/kg] vs IR; + P <0.05 for IR+BPS [100 µg/kg] vs IR; ^ P <0.05 for IR+BPS [100 µg/kg] vs IR+BPS [50 µg/kg]). Abbreviations: BPS, beraprost sodium; IL-1β, interleukin-1β; IODs, integrated optical densities; IR, ischemia-reperfusion; TNF-α, tumor necrosis factor-α; qRT-PCR, quantitative real-time polymerase chain reaction.

Article Snippet: IL-1β and TNF-α levels were determined by ELISA kits purchased from Anogen-Yes Biotech Laboratories Ltd. (Ontario, Canada).

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Staining, Software, Western Blot, Real-time Polymerase Chain Reaction